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Western blot analysis of extracts of various cell lines, using CXCL13 antibody (16-229) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Western blot analysis of extracts of various cell lines, using CXCL13 antibody (16-229) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

CXCL13 Antibody

CATALOG NUMBER: 16-229

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CXCL13 (NP_006410.1).
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
WB: 1:500 - 1:2000
Positive Control 1:
Mouse spleen
Positive Control 2:
Mouse thymus
Positive Control 3:
Mouse liver
Predicted Molecular Weight:
Observed: 14kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CXCL13
Additional Names:
CXCL13, SCYB13, chemokine (C-X-C motif) ligand 13, chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant), B-lymphocyte chemoattractant, BLR1L, BLC, B-cell-homing chemokine (ligand for Burkitt's lymphoma receptor-1), B-cell-attracting chemokine 1, B-cell chemoattractant, BCA-1, BCA1, ANGIE2, ANGIE
Ncbi Gene Id Number:
10563
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
B lymphocyte chemoattractant, independently cloned and named Angie, is an antimicrobial peptide and CXC chemokine strongly expressed in the follicles of the spleen, lymph nodes, and Peyer's patches. It preferentially promotes the migration of B lymphocytes (compared to T cells and macrophages), apparently by stimulating calcium influx into, and chemotaxis of, cells expressing Burkitt's lymphoma receptor 1 (BLR-1). It may therefore function in the homing of B lymphocytes to follicles.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

16-229

List Size:
100 uL

List Price:

$419.00

Out of stock

Datasheet
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