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Western blot analysis of extracts of various cell lines, using ZNRF3 antibody (16-383) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human colon carcinoma using ZNRF3 antibody (16-383) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using ZNRF3 antibody (16-383) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver using ZNRF3 antibody (16-383) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using ZNRF3 antibody (16-383) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat lung using ZNRF3 antibody (16-383) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using ZNRF3 antibody (16-383) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.

ZNRF3 Antibody

CATALOG NUMBER: 16-383

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ZNRF3 (NP_115549.2).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
HT-29
Positive Control 2:
HepG2
Positive Control 3:
Mouse testis
Predicted Molecular Weight:
Observed: 120kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
ZNRF3
Additional Names:
BK747E2.3, RNF203, E3 ubiquitin-protein ligase ZNRF3, CTA-292E10.6, RING finger protein 203, RING-type E3 ubiquitin transferase ZNRF3, novel C3HC4 type Zinc finger (ring finger), zinc/RING finger protein 3
Ncbi Gene Id Number:
84133
User Note:
Optimal dilutions for each application to be determined by the researcher.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

16-383

List Size:
100 uL

List Price:

$436.00

Datasheet
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