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Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K27 antibody (16-486) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K27 antibody (16-486) at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded human colon carcinoma using TriMethyl-Histone H3-K27 antibody (16-486) at dilution of 1:25 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using TriMethyl-Histone H3-K27 antibody (16-486) at dilution of 1:25 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using TriMethyl-Histone H3-K27 antibody (16-486) at dilution of 1:25 (40x lens).
Immunofluorescence analysis of C6 cells using TriMethyl-Histone H3-K27 antibody (16-486) at dilution of 1:25. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using TriMethyl-Histone H3-K27 antibody (16-486) at dilution of 1:25. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2OS cells using TriMethyl-Histone H3-K27 antibody (16-486) at dilution of 1:25. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K27 antibody (16-486) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.

TriMethyl-Histone H3-K27 mAb

CATALOG NUMBER: 16-486

Clonality:
Monoclonal
Tested Applications:
ChIP, IF, IHC, IP, WB
Host Species:
Mouse
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic methylated peptide corresponding to residues surrounding K27 of human histone H3
Conjugate:
Unconjugated
Tested Applications:
ChIP, IF, IHC, IP, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
IP: 1:50 - 1:200
ChIP: 1:20 - 1:100
Purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
H3K27me3
Additional Names:
H3F3A, H3t, H3.4, H3/g, H3FT
Ncbi Gene Id Number:
8290
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

16-486

List Size:
100 uL

List Price:

$540.00

Datasheet
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