Western blot analysis of extracts of various cell lines, using Apoe antibody (16-562) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.

Western blot analysis of extracts of various cell lines, using Apoe antibody (16-562) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

Apoe Antibody

CATALOG NUMBER: 16-562

Clonality:

Polyclonal

Tested Applications:

IF, WB

Host Species:

Rabbit

Species Reactivity:

Human, Mouse

Conjugate:

Unconjugated

Specifications

Host Species:

Rabbit

Species Reactivity:

Human, Mouse

Immunogen:

A synthetic peptide of mouse Apoe

Conjugate:

Unconjugated

Tested Applications:

IF, WB

Application Note:

WB: 1:500 - 1:2000
IF: 1:50 - 1:200

Positive Control 1:

mouse brain

Positive Control 2:

mouse liver

Predicted Molecular Weight:

Observed: 36kDa

Advanced Validation

Properties

Purification:

Affinity purification

Clonality:

Polyclonal

Isotype:

IgG

Physical State:

Liquid

Buffer:

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Concentration:

batch dependent

Storage Conditions:

Store at -20°C. Avoid freeze / thaw cycles.

Additional Info

Ncbi Official Symbol:

Apoe

Additional Names:

APO-E Antibody: AD2, LPG, LDLCQ5, Apolipoprotein E, Apo-E

Ncbi Gene Id Number:

11816

User Note:

Optimal dilutions for each application to be determined by the researcher.

Background

Citations

Set Contents

Disclaimer

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

16-562

List Size:

50 uL

List Price:

$309.00

Out of stock

Datasheet

Shipping Info

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Apoe Antibody

Clonality:

Tested Applications:

Host Species:

Species Reactivity:

Conjugate:

Host Species:

Species Reactivity:

Immunogen:

Conjugate:

Tested Applications:

Application Note:

Positive Control 1:

Positive Control 2:

Predicted Molecular Weight:

Purification:

Clonality:

Isotype:

Physical State:

Buffer:

Concentration:

Storage Conditions:

Ncbi Official Symbol:

Additional Names:

Ncbi Gene Id Number:

User Note:

Disclaimer:

List Size:

Shipping Info

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