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Western blot analysis of extracts of various cell lines, using XRCC4 antibody (16-747) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human appendix using XRCC4 antibody (16-747) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using XRCC4 antibody (16-747) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using XRCC4 antibody (16-747) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of 293T cells using XRCC4 antibody (16-747) at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using XRCC4 antibody (16-747) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

XRCC4 Antibody

CATALOG NUMBER: 16-747

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-336 of human XRCC4 (NP_071801.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:10 - 1:100
Positive Control 1:
Jurkat
Positive Control 2:
MCF7
Positive Control 3:
K562
Positive Control 4:
HeLa
Positive Control 5:
mouse thymus
Positive Control 6:
mouse heart
Predicted Molecular Weight:
Observed: 55kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
XRCC4
Additional Names:
DNA repair protein XRCC4, X-ray repair cross-complementing protein 4, XRCC4
Ncbi Gene Id Number:
7518
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene functions together with DNA ligase IV and the DNA-dependent protein kinase in the repair of DNA double-strand breaks. This protein plays a role in both non-homologous end joining and the completion of V(D)J recombination. Mutations in this gene can cause short stature, microcephaly, and endocrine dysfunction (SSMED). Alternative splicing generates several transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

16-747

List Size:
100 uL

List Price:

$436.00

Datasheet
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