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Western blot analysis of extracts of various cell lines, using AKR1C3 antibody (18-113) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 20s.
Immunoprecipitation analysis of 200ug extracts of K-562 cells, using 3 ug AKR1C3 antibody (18-113). Western blot was performed from the immunoprecipitate using AKR1C3 antibody (18-113) at a dilition of 1:1000.
Western blot analysis of extracts of various cell lines, using AKR1C3 antibody (18-113) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 20s.

AKR1C3 Antibody

CATALOG NUMBER: 18-113

Clonality:
Polyclonal
Tested Applications:
IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-323 of human AKR1C3 (NP_003730.4).
Conjugate:
Unconjugated
Tested Applications:
IP, WB
Application Note:
WB: 1:500 - 1:2000
IP: 1:50 - 1:100
Positive Control 1:
U-87MG
Positive Control 2:
293T
Positive Control 3:
LO2
Positive Control 4:
A-549
Positive Control 5:
DU145
Positive Control 6:
Mouse kidney
Predicted Molecular Weight:
Observed: 37kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
AKR1C3
Additional Names:
AKR1C3, DD3, HAKRB, HAKRe, HA1753, HSD17B5, hluPGFS, KIAA0119, aldo-keto reductase family 1, member C3 (3-alpha hydroxysteroid dehydrogenase, type II), prostaglandin F synthase, dihydrodiol dehydrogenase 3, chlordecone reductase homolog, hydroxysteroid (17-beta) dehydrogenase 5, type IIb 3-alpha hydroxysteroid dehydrogenase, trans-1, 2-dihydrobenzene-1, 2-diol dehydrogenase, DDH1, PGFS
Ncbi Gene Id Number:
8644
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ), and the oxidation of 9alpha,11beta-PGF2 to PGD2. It may play an important role in the pathogenesis of allergic diseases such as asthma, and may also have a role in controlling cell growth and/or differentiation. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. Three transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

18-113

List Size:
100 uL

List Price:

$436.00

Datasheet
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