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Western blot analysis of extracts of various cell lines, using SMARCC2 antibody (18-417) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat kidney using SMARCC2 antibody (18-417) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate cancer using SMARCC2 antibody (18-417) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using SMARCC2 antibody (18-417) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 60s.

SMARCC2 Antibody

CATALOG NUMBER: 18-417

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 150-250 of human SMARCC2 (NP_620706.1).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:200 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
U-87MG
Positive Control 2:
Mouse spinal cord
Predicted Molecular Weight:
Observed: 171kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
SMARCC2
Additional Names:
BAF17CRACC2, CSS8, Rsc8, SWI/SNF complex subunit SMARCC2, SWI/SNF complex 170 kDa subunit, SWI3-like protein, chromatin remodeling complex BAF170 subunit, mammalian chromatin remodeling complex BRG1-associated factor 170
Ncbi Gene Id Number:
6601
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and contains a predicted leucine zipper motif typical of many transcription factors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

18-417

List Size:
100 uL

List Price:

$436.00

Datasheet
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