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Western blot analysis of extracts of various cell lines, using THY1 antibody (18-520) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Immunofluorescence analysis of Mouse brain cells using THY1 antibody (18-520) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of Rat brain cells using THY1 antibody (18-520) at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using THY1 antibody (18-520) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.

THY1 Antibody

CATALOG NUMBER: 18-520

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 20-130 of human THY1 (NP_006279.2).
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
WB: 1:1000 - 1:2000
IF: 1:50 - 1:200
Positive Control 1:
Mouse brain
Positive Control 2:
Mouse kidney
Positive Control 3:
Mouse lung
Positive Control 4:
Rat brain
Positive Control 5:
Rat kidney
Predicted Molecular Weight:
Observed: 25kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
THY1
Additional Names:
Thy-1 membrane glycoprotein, CDw90, Thy-1 antigen, CD90, THY1
Ncbi Gene Id Number:
7070
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a cell surface glycoprotein and member of the immunoglobulin superfamily of proteins. The encoded protein is involved in cell adhesion and cell communication in numerous cell types, but particularly in cells of the immune and nervous systems. The encoded protein is widely used as a marker for hematopoietic stem cells. This gene may function as a tumor suppressor in nasopharyngeal carcinoma. Alternative splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

18-520

List Size:
100 uL

List Price:

$436.00

Datasheet
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