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Western blot analysis of extracts of HL-60 cells, using XRCC3 antibody (18-524) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat testis using XRCC3 antibody (18-524) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using XRCC3 antibody (18-524) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using XRCC3 antibody (18-524) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using XRCC3 antibody (18-524). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of HL-60 cells, using XRCC3 antibody (18-524) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

XRCC3 Antibody

CATALOG NUMBER: 18-524

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 62-346 of human XRCC3 (NP_001093588.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
HL-60
Predicted Molecular Weight:
Observed: 37kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
XRCC3
Additional Names:
DNA repair protein XRCC3, X-ray repair cross-complementing protein 3, CMM6
Ncbi Gene Id Number:
7517
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the RecA/Rad51-related protein family that participates in homologous recombination to maintain chromosome stability and repair DNA damage. This gene functionally complements Chinese hamster irs1SF, a repair-deficient mutant that exhibits hypersensitivity to a number of different DNA-damaging agents and is chromosomally unstable. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. Alternatively spliced transcript variants encoding the same protein have been identified.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

18-524

List Size:
100 uL

List Price:

$436.00

Datasheet
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