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Western blot analysis of extracts of HeLa cells, using KRT7 antibody (18-719).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human placenta using KRT7 antibody (18-719) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of human lung cancer cells using KRT7 antibody (18-719) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of rat bronchus cells using KRT7 antibody (18-719) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse bronchus cells using KRT7 antibody (18-719) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of human lung cancer cells using KRT7 antibody (18-719) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of rat bronchus cells using KRT7 antibody (18-719) at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of HeLa cells, using KRT7 antibody (18-719).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

KRT7 Antibody

CATALOG NUMBER: 18-719

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 230-469 of human KRT7 (NP_005547.3).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
HeLa
Predicted Molecular Weight:
Observed: 51kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
KRT7
Additional Names:
Keratin, type II cytoskeletal 7, Cytokeratin-7, CK-7, Keratin-7, K7, Sarcolectin, Type-II keratin Kb7, KRT7, SCL
Ncbi Gene Id Number:
3855
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

18-719

List Size:
100 uL

List Price:

$436.00

Datasheet
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