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Western blot analysis of extracts of various cell lines, using SDHA antibody (18-730) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 3s.
Immunohistochemistry of paraffin-embedded rat heart using SDHA antibody (18-730) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using SDHA antibody (18-730) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophageal cancer using SDHA antibody (18-730) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using SDHA antibody (18-730). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using SDHA antibody (18-730) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.

SDHA Antibody

CATALOG NUMBER: 18-730

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 385-664 of human SDHA (NP_004159.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:10 - 1:100
Positive Control 1:
HepG2
Positive Control 2:
U-251MG
Positive Control 3:
PC-3
Positive Control 4:
MCF7
Positive Control 5:
Mouse liver
Positive Control 6:
Mouse stomach
Predicted Molecular Weight:
Observed: 70kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
SDHA
Additional Names:
Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial, Flavoprotein subunit of complex II, Fp, SDHA, SDH2, SDHF
Ncbi Gene Id Number:
6389
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations in this gene have been associated with a form of mitochondrial respiratory chain deficiency known as Leigh Syndrome. A pseudogene has been identified on chromosome 3q29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

18-730

List Size:
100 uL

List Price:

$436.00

Datasheet
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