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Western blot analysis of extracts of various cell lines, using USO1 antibody (18-816) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human tonsil using USO1 antibody (18-816) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using USO1 antibody (18-816) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using USO1 antibody (18-816). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using USO1 antibody (18-816) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

USO1 Antibody

CATALOG NUMBER: 18-816

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
A synthetic peptide of human USO1
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
22Rv1
Positive Control 2:
BT-474
Positive Control 3:
K-562
Positive Control 4:
THP-1
Positive Control 5:
293T
Positive Control 6:
A-549
Predicted Molecular Weight:
Observed: 115kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
USO1
Additional Names:
vesicle docking protein p115, transcytosis associated protein, USO1 homolog, vesicle docking protein, vesicle docking protein (yeast), USO1, transcytosis-associated protein, TAP, P115, VDP p115, VDP
Ncbi Gene Id Number:
8615
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase. It is regulated by phosphorylation: dephosphorylated protein associates with the Golgi membrane and dissociates from the membrane upon phosphorylation. Ras-associated protein 1 recruits this protein to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where it interacts with a set of COPII vesicle-associated SNAREs to form a cis-SNARE complex that promotes targeting to the Golgi apparatus. Alternative splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

18-816

List Size:
100 uL

List Price:

$436.00

Datasheet
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