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Western blot analysis of extracts of various cell lines, using TPM2 antibody (18-940) at 1:400 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat testis using TPM2 antibody (18-940) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using TPM2 antibody (18-940) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human rectal cancer using TPM2 antibody (18-940) at dilution of 1:200 (40x lens).
Western blot analysis of extracts of various cell lines, using TPM2 antibody (18-940) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

TPM2 Antibody

CATALOG NUMBER: 18-940

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic peptide of human TPM2
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:1000
IHC: 1:50 - 1:100
IF: 1:50 - 1:100
Positive Control 1:
SW620
Positive Control 2:
HeLa
Positive Control 3:
MCF7
Positive Control 4:
U-87MG
Positive Control 5:
Mouse skeletal muscle
Positive Control 6:
Mouse lung
Predicted Molecular Weight:
Observed: 36kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
TPM2
Additional Names:
TPM2, AMCD1, DA1, TMSB, DA2B, NEM4, HEL-S-273
Ncbi Gene Id Number:
7169
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes beta-tropomyosin, a member of the actin filament binding protein family, and mainly expressed in slow, type 1 muscle fibers. Mutations in this gene can alter the expression of other sarcomeric tropomyosin proteins, and cause cap disease, nemaline myopathy and distal arthrogryposis syndromes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

18-940

List Size:
100 uL

List Price:

$436.00

Datasheet
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