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Western blot analysis of extracts of various cell lines, using IGF2BP3 antibody (19-372) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
Immunohistochemistry of paraffin-embedded human esophagus using IGF2BP3 antibody (19-372) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using IGF2BP3 antibody (19-372) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using IGF2BP3 antibody (19-372) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of U2OS cells using IGF2BP3 antibody (19-372) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 100ug extracts of HepG2 cells using 3ug IGF2BP3 antibody (19-372). Western blot was performed from the immunoprecipitate using IGF2BP3 antibody (19-372) at a dilition of 1:1000.
Western blot analysis of extracts of various cell lines, using IGF2BP3 antibody (19-372) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.

IGF2BP3 Antibody

CATALOG NUMBER: 19-372

Clonality:
Polyclonal
Tested Applications:
IF, IHC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 300-579 of human IGF2BP3 (NP_006538.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, IP, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
IP: 1:20 - 1:50
Positive Control 1:
HepG2
Positive Control 2:
A-549
Positive Control 3:
293T
Positive Control 4:
HeLa
Predicted Molecular Weight:
Observed: 70kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
IGF2BP3
Additional Names:
CT98, IMP-3, IMP3, KOC, KOC1, VICKZ3, insulin-like growth factor 2 mRNA-binding protein 3, IGF-II mRNA-binding protein 3, IGF2 mRNA-binding protein 3, KH domain containing protein overexpressed in cancer, VICKZ family member 3, cancer/testis antigen 98
Ncbi Gene Id Number:
10643
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is primarily found in the nucleolus, where it can bind to the 5' UTR of the insulin-like growth factor II leader 3 mRNA and may repress translation of insulin-like growth factor II during late development. The encoded protein contains several KH domains, which are important in RNA binding and are known to be involved in RNA synthesis and metabolism. A pseudogene exists on chromosome 7, and there are putative pseudogenes on other chromosomes.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-372

List Size:
100 uL

List Price:

$436.00

Datasheet
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