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Western blot analysis of extracts of various cell lines, using VAPB antibody (19-617) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human liver cancer using VAPB Antibody (19-617) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using VAPB Antibody (19-617) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using VAPB Antibody (19-617) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of HeLa cells using VAPB antibody (19-617). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using VAPB antibody (19-617) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

VAPB Antibody

CATALOG NUMBER: 19-617

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 10-222 of human VAPB (NP_004729.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:20 - 1:50
Positive Control 1:
BT-474
Positive Control 2:
SW480
Positive Control 3:
HepG2
Positive Control 4:
NCI-H460
Positive Control 5:
Raji
Positive Control 6:
Mouse brain
Predicted Molecular Weight:
Observed: 35kDa/47kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
VAPB
Additional Names:
Vesicle-associated membrane protein-associated protein B/C, VAMP-B/VAMP-C, VAMP-associated protein B/C, VAP-B/VAP-C, VAPB
Ncbi Gene Id Number:
9217
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a type IV membrane protein found in plasma and intracellular vesicle membranes. The encoded protein is found as a homodimer and as a heterodimer with VAPA. This protein also can interact with VAMP1 and VAMP2 and may be involved in vesicle trafficking.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-617

List Size:
100 uL

List Price:

$436.00

Datasheet
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