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Western blot analysis of extracts of various cell lines, using SKIV2L2 antibody (19-742) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Immunohistochemistry of paraffin-embedded human colon using SKIV2L2 antibody (19-742) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using SKIV2L2 antibody (19-742) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using SKIV2L2 antibody (19-742) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using SKIV2L2 antibody (19-742) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.

SKIV2L2 Antibody

CATALOG NUMBER: 19-742

Clonality:
Polyclonal
Tested Applications:
IHC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-304 of human SKIV2L2 (NP_056175.3).
Conjugate:
Unconjugated
Tested Applications:
IHC, IP, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IP: 1:50 - 1:200
Positive Control 1:
HeLa
Positive Control 2:
22RV1
Positive Control 3:
293T
Positive Control 4:
U-87MG
Positive Control 5:
Mouse testis
Positive Control 6:
Mouse spleen
Predicted Molecular Weight:
Observed: 123kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
SKIV2L2
Additional Names:
Superkiller viralicidic activity 2-like 2, ATP-dependent RNA helicase SKIV2L2, TRAMP-like complex helicase, SKIV2L2, KIAA0052, Mtr4
Ncbi Gene Id Number:
23517
User Note:
Optimal dilutions for each application to be determined by the researcher.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-742

List Size:
100 uL

List Price:

$436.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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