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Western blot analysis of extracts of various cell lines, using CSDE1 antibody (19-954) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunoprecipitation analysis of 100ug extracts of HeLa cells using 3ug CSDE1 antibody (19-954). Western blot was performed from the immunoprecipitate using CSDE1 antibody (19-954) at a dilition of 1:1000.
Western blot analysis of extracts of various cell lines, using CSDE1 antibody (19-954) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

CSDE1 Antibody

CATALOG NUMBER: 19-954

Clonality:
Polyclonal
Tested Applications:
IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 369-600 of human CSDE1 (NP_009089.4).
Conjugate:
Unconjugated
Tested Applications:
IP, WB
Application Note:
WB: 1:500 - 1:2000
IP: 1:20 - 1:50
Positive Control 1:
U-87MG
Positive Control 2:
LO2
Positive Control 3:
HeLa
Positive Control 4:
MCF7
Positive Control 5:
Mouse brain
Predicted Molecular Weight:
Observed: 102kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CSDE1
Additional Names:
Cold shock domain-containing protein E1, N-ras upstream gene protein, Protein UNR, CSDE1, D1S155E, KIAA0885, NRU, UNR
Ncbi Gene Id Number:
7812
User Note:
Optimal dilutions for each application to be determined by the researcher.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-954

List Size:
100 uL

List Price:

$540.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

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Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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