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Western blot analysis of extracts of various cell lines, using AAAS antibody (22-213) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat brain using AAAS Antibody (22-213) at dilution of 1:100 (20x lens).
Immunohistochemistry of paraffin-embedded human appendicitis using AAAS Antibody (22-213) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using AAAS Antibody (22-213) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using AAAS Antibody (22-213) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using AAAS Antibody (22-213) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using AAAS antibody (22-213). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using AAAS antibody (22-213) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

AAAS Antibody

CATALOG NUMBER: 22-213

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 287-546 of human AAAS (NP_056480.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
MCF7
Positive Control 2:
HeLa
Positive Control 3:
293T
Predicted Molecular Weight:
Observed: 59kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
AAAS
Additional Names:
Aladin, Adracalin, AAAS, ADRACALA
Ncbi Gene Id Number:
8086
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a member of the WD-repeat family of regulatory proteins and may be involved in normal development of the peripheral and central nervous system. The encoded protein is part of the nuclear pore complex and is anchored there by NDC1. Defects in this gene are a cause of achalasia-addisonianism-alacrima syndrome (AAAS), also called triple-A syndrome or Allgrove syndrome. Two transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-213

List Size:
100 uL

List Price:

$436.00

Datasheet
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