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Western blot analysis of extracts of various cell lines, using CALU antibody (22-295) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit._Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse skin using CALU antibody (22-295) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat lung using CALU antibody (22-295) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using CALU antibody (22-295) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of NIH-3T3 cells using CALU antibody (22-295) at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using CALU antibody (22-295) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit._Exposure time: 90s.

CALU Antibody

CATALOG NUMBER: 22-295

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 20-315 of human CALU (NP_001210.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
IF: 1:50 - 1:200
Positive Control 1:
NCI-H460
Positive Control 2:
Mouse lung
Predicted Molecular Weight:
Observed: 50kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CALU
Additional Names:
Calumenin, Crocalbin, IEF SSP 9302, CALU
Ncbi Gene Id Number:
813
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The product of this gene is a calcium-binding protein localized in the endoplasmic reticulum (ER) and it is involved in such ER functions as protein folding and sorting. This protein belongs to a family of multiple EF-hand proteins (CERC) that include reticulocalbin, ERC-55, and Cab45 and the product of this gene. Alternatively spliced transcript variants encoding different isoforms have been identified.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-295

List Size:
100 uL

List Price:

$436.00

Datasheet
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