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Western blot analysis of extracts of various cell lines, using DDX1 antibody (22-324) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat brain using DDX1 Antibody (22-324) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using DDX1 Antibody (22-324) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using DDX1 Antibody (22-324) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using DDX1 Antibody (22-324) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF7 cells using DDX1 antibody (22-324). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 100ug extracts of 293T cells using 3ug DDX1 antibody (22-324). Western blot was performed from the immunoprecipitate using DDX1 antibody (22-324) at a dilition of 1:1000.
Western blot analysis of extracts of various cell lines, using DDX1 antibody (22-324) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

DDX1 Antibody

CATALOG NUMBER: 22-324

Clonality:
Polyclonal
Tested Applications:
IF, IHC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 441-740 of human DDX1 (NP_004930.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, IP, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:10 - 1:100
IP: 1:20 - 1:50
Positive Control 1:
MCF7
Positive Control 2:
HepG2
Positive Control 3:
HeLa
Positive Control 4:
293T
Positive Control 5:
U-251MG
Positive Control 6:
Mouse brain
Predicted Molecular Weight:
Observed: 100kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
DDX1
Additional Names:
DDX1, DBP-RB, UKVH5d
Ncbi Gene Id Number:
1653
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein of unknown function. It shows high transcription levels in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-324

List Size:
100 uL

List Price:

$436.00

Datasheet
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