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Western blot analysis of extracts of various cell lines, using PUF60 antibody (22-425) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human well-differentiated squamous skin carcinoma using PUF60 antibody (22-425) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using PUF60 antibody (22-425). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 150ug extracts of A549 cells using 3ug PUF60 antibody (22-425). Western blot was performed from the immunoprecipitate using PUF60 antibody (22-425) at a dilition of 1:500.
Western blot analysis of extracts of various cell lines, using PUF60 antibody (22-425) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

PUF60 Antibody

CATALOG NUMBER: 22-425

Clonality:
Polyclonal
Tested Applications:
IF, IHC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 243-542 of human PUF60 (NP_055096.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, IP, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:100
IP: 1:20 - 1:50
Positive Control 1:
THP-1
Positive Control 2:
A-549
Positive Control 3:
Mouse brain
Positive Control 4:
Mouse thymus
Positive Control 5:
Mouse spleen
Positive Control 6:
Rat lung
Predicted Molecular Weight:
Observed: 65kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
PUF60
Additional Names:
PUF60, FIR, FLJ31379, RoBPI, SIAHBP1, VRJS
Ncbi Gene Id Number:
22827
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a nucleic acid-binding protein that plays a role in a variety of nuclear processes, including pre-mRNA splicing and transcriptional regulation. The encoded protein forms a complex with the far upstream DNA element (FUSE) and FUSE-binding protein at the myelocytomatosis oncogene (MYC) promoter. This complex represses MYC transcription through the core-TFIIH basal transcription factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-425

List Size:
100 uL

List Price:

$436.00

Datasheet
Shipping Info

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