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Western blot analysis of extracts of various cell lines, using RBP2 antibody (22-433) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Immunohistochemistry of paraffin-embedded human liver damage using RBP2 antibody (22-433) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using RBP2 antibody (22-433) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using RBP2 antibody (22-433) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF-7 cells using RBP2 antibody (22-433). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using RBP2 antibody (22-433) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.

RBP2 Antibody

CATALOG NUMBER: 22-433

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-134 of human RBP2 (NP_004155.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:100
Positive Control 1:
Mouse small intestine
Positive Control 2:
Rat liver
Predicted Molecular Weight:
Observed: 15-17kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
RBP2
Additional Names:
Retinol-binding protein 2, Cellular retinol-binding protein II, CRBP-II, RBP2, CRBP2
Ncbi Gene Id Number:
5948
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes an abundant protein present in the small intestinal epithelium. It is thought to participate in the uptake and/or intracellular metabolism of vitamin A. Vitamin A is a fat-soluble vitamin necessary for growth, reproduction, differentiation of epithelial tissues, and vision. This protein may also modulate the supply of retinoic acid to the nuclei of endometrial cells during the menstrual cycle.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-433

List Size:
100 uL

List Price:

$436.00

Datasheet
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