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Western blot analysis of extracts of various cell lines, using SARS antibody (22-446) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat spinal cord using SARS Antibody (22-446) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using SARS Antibody (22-446) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF7 cells using SARS antibody (22-446). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using SARS antibody (22-446) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

SARS Antibody

CATALOG NUMBER: 22-446

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human SARS (NP_006504.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
MCF7
Positive Control 2:
HepG2
Positive Control 3:
HeLa
Positive Control 4:
U-251MG
Positive Control 5:
293T
Positive Control 6:
Mouse kidney
Predicted Molecular Weight:
Observed: 59kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
SARS
Additional Names:
SARS, SERS, SERRS
Ncbi Gene Id Number:
6301
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene belongs to the class II amino-acyl tRNA family. The encoded enzyme catalyzes the transfer of L-serine to tRNA (Ser) and is related to bacterial and yeast counterparts. Multiple alternatively spliced transcript variants have been described but the biological validity of all variants is unknown.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-446

List Size:
100 uL

List Price:

$436.00

Datasheet
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