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Western blot analysis of extracts of various cell lines, using TRAPPC10 antibody (22-478) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15min.
Immunofluorescence analysis of U2OS cells using TRAPPC10 antibody (22-478). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using TRAPPC10 antibody (22-478) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15min.

TRAPPC10 Antibody

CATALOG NUMBER: 22-478

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human TRAPPC10 (NP_003265.3).
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
WB: 1:500 - 1:2000
IF: 1:50 - 1:100
Positive Control 1:
Mouse kidney
Positive Control 2:
Rat lung
Predicted Molecular Weight:
Observed: 112kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
TRAPPC10
Additional Names:
EHOC-1, EHOC1, GT334, TMEM1, TRS13TRS3trafficking protein particle complex subunit 1TRAPP 130 kDa subunit, TRAPP subunit TMEM1, epilepsy holoprosencephaly candidate-1 protein, trafficking protein particle complex subunit 13trafficking protein particle complex subunit TMEM1, transmembrane protein 1, transport protein particle subunit TMEM1
Ncbi Gene Id Number:
7109
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a transmembrane protein found in the cis-Golgi complex. The encoded protein is part of the multisubunit transport protein particle (TRAPP) complex and may be involved in vesicular transport from the endoplasmic reticulum to the Golgi. Mutations in this gene could be responsible for the Unverricht-Lundborg type of progressive myoclonus epilepsy, or for autoimmune polyglandular disease type 1.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-478

List Size:
100 uL

List Price:

$436.00

Datasheet
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