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Western blot analysis of extracts of various cell lines, using TSHR antibody (22-481) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using TSHR Antibody (22-481) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using TSHR Antibody (22-481) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using TSHR Antibody (22-481) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using TSHR antibody (22-481) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

TSHR Antibody

CATALOG NUMBER: 22-481

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 21-253 of human TSHR (NP_001018046.1).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
HeLa
Positive Control 2:
SKOV3
Positive Control 3:
OVCAR3
Positive Control 4:
mouse ovary
Positive Control 5:
mouse thymus
Positive Control 6:
mouse eye
Predicted Molecular Weight:
Observed: 110kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
TSHR
Additional Names:
TSHR, LGR3, MGC75129, hTSHR-I, CHNG1
Ncbi Gene Id Number:
7253
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a membrane protein and a major controller of thyroid cell metabolism. The encoded protein is a receptor for thyrothropin and thyrostimulin, and its activity is mediated by adenylate cyclase. Defects in this gene are a cause of several types of hyperthyroidism. Three transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-481

List Size:
100 uL

List Price:

$436.00

Datasheet
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