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Western blot analysis of extracts of various cell lines, using XPO5 antibody (22-488).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human oophoroma using XPO5 antibody (22-488) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse jejunum using XPO5 antibody (22-488) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF7 cells using XPO5 antibody (22-488).
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug XPO5 antibody (22-488). Western blot was performed from the immunoprecipitate using XPO5 antibody (22-488) at a dilition of 1:1000.
Western blot analysis of extracts of various cell lines, using XPO5 antibody (22-488).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

XPO5 Antibody

CATALOG NUMBER: 22-488

Clonality:
Polyclonal
Tested Applications:
IF, IHC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 925-1204 of human XPO5 (NP_065801.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, IP, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:100
IP: 1:50 - 1:200
Positive Control 1:
MCF7
Positive Control 2:
U-251MG
Positive Control 3:
NIH/3T3
Positive Control 4:
HeLa
Positive Control 5:
PC-12
Positive Control 6:
Mouse brain
Predicted Molecular Weight:
Observed: 125kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
XPO5
Additional Names:
Exportin-5, Exp5, Ran-binding protein 21, XPO5, KIAA1291, RANBP21
Ncbi Gene Id Number:
57510
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the karyopherin family that is required for the transport of small RNAs and double-stranded RNA-binding proteins from the nucleus to the cytoplasm. The encoded protein translocates cargo through the nuclear pore complex in a RanGTP-dependent process.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-488

List Size:
100 uL

List Price:

$436.00

Datasheet
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