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Western blot analysis of extracts of various cell lines, using Bcl9 antibody (22-491) at 1:400 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat brain using BCL9 antibody (22-491) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using BCL9 antibody (22-491) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using Bcl9 antibody (22-491) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

Bcl9 Antibody

CATALOG NUMBER: 22-491

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein of mouse Bcl9
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
MCF7
Positive Control 2:
Mouse pancreas
Predicted Molecular Weight:
Observed: 160kDa/170kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
BCL9
Additional Names:
2610202E01Rik, 8030475K17Rik, A330041G23Rik, Gm13B-cell CLL/lymphoma 9 protein, B-cell lymphoma 9 protein, bcl-9, nuclear co-factor of beta-catenin signalling
Ncbi Gene Id Number:
77578
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
BCL9 is associated with B-cell acute lymphoblastic leukemia. It may be a target of translocation in B-cell malignancies with abnormalities of 1q21. Its function is unknown. The overexpression of BCL9 may be of pathogenic significance in B-cell malignancies.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-491

List Size:
100 uL

List Price:

$436.00

Datasheet
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