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Western blot analysis of extracts of various cell lines, using ZNF148 Antibody (22-622) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat lung using ZNF148 antibody (22-622) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat liver using ZNF148 antibody (22-622) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using ZNF148 antibody (22-622) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using ZNF148 antibody (22-622) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung using ZNF148 antibody (22-622) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using ZNF148 antibody (22-622) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human appendix using ZNF148 antibody (22-622) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human smooth muscle using ZNF148 antibody (22-622) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using NASP Antibody (22-569) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using ZNF148 antibody (22-622) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using ZNF148 antibody (22-622) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using ZNF148 antibody (22-622) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using ZNF148 antibody (22-622) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A-549 cells using ZNF148 antibody (22-622).
Western blot analysis of extracts of various cell lines, using ZNF148 Antibody (22-622) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.

ZNF148 Antibody

CATALOG NUMBER: 22-622

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 690-794 of human ZNF148 (NP_068799.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:1000
IHC: 1:50 - 1:100
IF: 1:20 - 1:100
Positive Control 1:
K-562
Positive Control 2:
Mouse lung
Positive Control 3:
Rat lung
Predicted Molecular Weight:
Observed: 105kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
ZNF148
Additional Names:
Zinc finger protein 148, Transcription factor ZBP-89, Zinc finger DNA-binding protein 89, ZNF148, ZBP89
Ncbi Gene Id Number:
7707
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a member of the Kruppel family of zinc finger DNA binding proteins. The encoded protein activates transcription of the T-cell receptor and intestinal alkaline phosphatase genes but represses transcription of the ornithine decarboxylase, vimentin, gastrin, stomelysin, and enolase genes. Increased expression of this gene results in decreased patient survival rates from colorectal cancer, while mutations in this gene have been associated with global developmental delay, hypoplastic corpus callosum, and dysmorphic facies.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-622

List Size:
100 uL

List Price:

$436.00

Datasheet
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