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Western blot analysis of extracts of various cell lines, using EFTUD2 antibody (22-651) at 1:2000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 20s.
Immunohistochemistry of paraffin-embedded rat liver using EFTUD2 Antibody (22-651) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using EFTUD2 Antibody (22-651) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using EFTUD2 Antibody (22-651) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using EFTUD2 Antibody (22-651) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using EFTUD2 Antibody (22-651) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using EFTUD2 antibody (22-651).
Immunoprecipitation analysis of 100ug extracts of 293T cells using 3ug EFTUD2 antibody (22-651). Western blot was performed from the immunoprecipitate using EFTUD2 antibody (22-651) at a dilition of 1:1000.
Western blot analysis of extracts of various cell lines, using EFTUD2 antibody (22-651) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 20s.

EFTUD2 Antibody

CATALOG NUMBER: 22-651

Clonality:
Polyclonal
Tested Applications:
IF, IHC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-205 of human EFTUD2 (NP_004238.3).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, IP, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:100
IP: 1:20 - 1:50
Positive Control 1:
Jurkat
Positive Control 2:
HepG2
Positive Control 3:
HeLa
Positive Control 4:
Mouse testis
Positive Control 5:
Mouse brain
Positive Control 6:
Mouse spleen
Predicted Molecular Weight:
Observed: 120kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
EFTUD2
Additional Names:
116 kDa U5 small nuclear ribonucleoprotein component, Elongation factor Tu GTP-binding domain-containing protein 2, SNU114 homolog, hSNU114, U5 snRNP-specific protein, 116 kDa, U5-116 kDa, EFTUD2, KIAA0031, SNRP116
Ncbi Gene Id Number:
9343
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a GTPase which is a component of the spliceosome complex which processes precursor mRNAs to produce mature mRNAs. Mutations in this gene are associated with mandibulofacial dysostosis with microcephaly. Multiple transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-651

List Size:
100 uL

List Price:

$436.00

Datasheet
Shipping Info

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