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Western blot analysis of extracts of various cell lines, using ARFGAP1 antibody (22-715) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 3s.
Immunohistochemistry of paraffin-embedded rat kidney using ARFGAP1 antibody (22-715) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using ARFGAP1 antibody (22-715) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF7 cells using ARFGAP1 antibody (22-715). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using ARFGAP1 antibody (22-715) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.

ARFGAP1 Antibody

CATALOG NUMBER: 22-715

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human ARFGAP1 (NP_001268411.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:100
Positive Control 1:
BT-474
Positive Control 2:
HeLa
Positive Control 3:
HepG2
Positive Control 4:
MCF7
Positive Control 5:
Human placenta
Positive Control 6:
Mouse liver
Predicted Molecular Weight:
Observed: 45kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
ARFGAP1
Additional Names:
ADP-ribosylation factor GTPase-activating protein 1, ARF GAP 1, ADP-ribosylation factor 1 GTPase-activating protein, ARF1 GAP, ARF1-directed GTPase-activating protein, ARFGAP1, ARF1GAP
Ncbi Gene Id Number:
55738
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a GTPase-activating protein, which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Alternative splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-715

List Size:
100 uL

List Price:

$436.00

Datasheet
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