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Western blot analysis of extracts of various cell lines, using GTF2H3 antibody (22-768) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunofluorescence analysis of A549 cells using GTF2H3 antibody (22-768). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of HT-29 cells using 1ug GTF2H3 antibody (22-768). Western blot was performed from the immunoprecipitate using GTF2H3 antibody (22-768) at a dilition of 1:1000.
Western blot analysis of extracts of various cell lines, using GTF2H3 antibody (22-768) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

GTF2H3 Antibody

CATALOG NUMBER: 22-768

Clonality:
Polyclonal
Tested Applications:
IF, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-308 of human GTF2H3 (NP_001507.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IP, WB
Application Note:
WB: 1:500 - 1:2000
IF: 1:50 - 1:100
IP: 1:50 - 1:200
Positive Control 1:
HL-60
Positive Control 2:
HT-29
Positive Control 3:
MCF-7
Positive Control 4:
Jurkat
Predicted Molecular Weight:
Observed: 36kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
GTF2H3
Additional Names:
GTranscription Factor Antibodies2H3, P34, BTF2, TFB4, TFIIH
Ncbi Gene Id Number:
2967
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the TFB4 family. The encoded protein is a subunit of the core-TFIIH basal transcription factor and localizes to the nucleus. The encoded protein is involved in RNA transcription by RNA polymerase II and nucleotide excision repair and associates with the Cdk-activating kinase complex. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 14.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-768

List Size:
100 uL

List Price:

$436.00

Datasheet
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