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Western blot analysis of extracts of various cell lines, using Acetyl-Histone H3-K18 antibody (22-816) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Dot-blot analysis of all sorts of methylation peptides using Acetyl-Histone H3-K18 antibody (22-816) at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded rat ovary using H3K18ac antibody (22-816) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using H3K18ac antibody (22-816) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using H3K18ac antibody (22-816) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using H3K18ac antibody (22-816) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using H3K18ac antibody (22-816) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using H3K18ac antibody (22-816) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using Acetyl-Histone H3-K18 antibody (22-816) at dilution of 1:100.C6 cells were treated by TSA (1 uM) at 37 for 18 hours. Blue: DAPI for nuclear staining.
Immunohistochemistry of paraffin-embedded human stomach using LC3B antibody (22-775) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H3-K18 antibody (22-816) at dilution of 1:100.NIH/3T3 cells were treated by TSA (1 uM) at 37 for 18 hours. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using Acetyl-Histone H3-K18 antibody (22-816) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

Acetyl-Histone H3-K18 pAb

CATALOG NUMBER: 22-816

Clonality:
Polyclonal
Tested Applications:
ChIP, ChIPseq, IF, IHC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Immunogen:
A synthetic acetylated peptide corresponding to residues surrounding K18 of human H3
Conjugate:
Unconjugated
Tested Applications:
ChIP, ChIPseq, IF, IHC, IP, WB
Application Note:
WB: 1:1000 - 1:3000
IHC: 1:200 - 1:500
IF: 1:500 - 1:1000
IP: 1:200 - 1:500
CHIP: 1:50 - 1:100
CHIP seq: 1:50 - 1:100
Positive Control 1:
BT-474
Positive Control 2:
293T
Positive Control 3:
H3
Predicted Molecular Weight:
Observed: 16kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
H3K18ac
Additional Names:
H3/b, H3FB, ERBB, ERBB1, HER1, H3/a, H3/c, H3/d, H3/f, H3/h
Ncbi Gene Id Number:
8356
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-816

List Size:
100 uL

List Price:

$540.00

Datasheet
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