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Western blot analysis of extracts of various cell lines, using ASPSCR1 antibody (22-973) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat kidney using ASPSCR1 antibody (22-973) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using ASPSCR1 antibody (22-973) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using ASPSCR1 antibody (22-973) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using ASPSCR1 antibody (22-973) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using ASPSCR1 antibody (22-973) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using ASPSCR1 antibody (22-973) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using ASPSCR1 antibody (22-973) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of A-549 cells, using 3 ug ASPSCR1 antibody (22-973). Western blot was performed from the immunoprecipitate using ASPSCR1 antibody (22-973) at a dilition of 1:1000.
Western blot analysis of extracts of various cell lines, using ASPSCR1 antibody (22-973) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

ASPSCR1 Antibody

CATALOG NUMBER: 22-973

Clonality:
Polyclonal
Tested Applications:
IF, IHC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 284-553 of human ASPSCR1 (NP_076988.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, IP, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
IP: 1:50 - 1:100
Positive Control 1:
293T
Positive Control 2:
SW480
Positive Control 3:
A-549
Positive Control 4:
BT-474
Positive Control 5:
Mouse liver
Positive Control 6:
Mouse testis
Predicted Molecular Weight:
Observed: 80kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
ASPSCR1
Additional Names:
Tether containing UBX domain for GLUT4, Alveolar soft part sarcoma chromosomal region candidate gene 1 protein, Alveolar soft part sarcoma locus, Renal papillary cell carcinoma protein 17, UBX domain-containing protein 9, ASPSCR1, ASPL, RCC17, TUG, UBXD9, UBXN9
Ncbi Gene Id Number:
79058
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene contains a UBX domain and interacts with glucose transporter type 4 (GLUT4). This protein is a tether, which sequesters the GLUT4 in intracellular vesicles in muscle and fat cells in the absence of insulin, and redistributes the GLUT4 to the plasma membrane within minutes of insulin stimulation. Translocation t(X;17)(p11;q25) of this gene with transcription factor TFE3 gene results in a ASPSCR1-TFE3 fusion protein in alveolar soft part sarcoma and in renal cell carcinomas. Multiple alternatively spliced transcript variants have been found.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-973

List Size:
100 uL

List Price:

$436.00

Datasheet
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