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Western blot analysis of extracts of various cell lines, using TBL1XR1 antibody (23-215) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human oophoroma using TBL1XR1 antibody (23-215) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human brain cancer using TBL1XR1 antibody (23-215) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney using TBL1XR1 antibody (23-215) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using TBL1XR1 antibody (23-215) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

TBL1XR1 Antibody

CATALOG NUMBER: 23-215

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human TBL1XR1 (NP_078941.2).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
Jurkat
Positive Control 2:
HepG2
Positive Control 3:
NCI-H460
Positive Control 4:
THP-1
Positive Control 5:
Mouse brain
Positive Control 6:
Mouse spleen
Predicted Molecular Weight:
Observed: 60kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
TBL1XR1
Additional Names:
F-box-like/WD repeat-containing protein TBL1XR1, Nuclear receptor corepressor/HDAC3 complex subunit TBLR1, TBL1-related protein 1, Transducin beta-like 1X-related protein 1, TBL1XR1, IRA1, TBLR1
Ncbi Gene Id Number:
79718
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene is a member of the WD40 repeat-containing gene family and shares sequence similarity with transducin (beta)-like 1X-linked (TBL1X). The protein encoded by this gene is thought to be a component of both nuclear receptor corepressor (N-CoR) and histone deacetylase 3 (HDAC 3) complexes, and is required for transcriptional activation by a variety of transcription factors. Mutations in these gene have been associated with some autism spectrum disorders, and one finding suggests that haploinsufficiency of this gene may be a cause of intellectual disability with dysmorphism. Mutations in this gene as well as recurrent translocations involving this gene have also been observed in some tumors.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-215

List Size:
100 uL

List Price:

$436.00

Datasheet
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