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Western blot analysis of extracts of various cell lines, using ANKRD28 antibody (23-537) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Western blot analysis of extracts of various cell lines, using ANKRD28 antibody (23-537) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

ANKRD28 Antibody

CATALOG NUMBER: 23-537

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human ANKRD28 (NP_056014.2).
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
WB: 1:500 - 1:2000
Positive Control 1:
U-251MG
Positive Control 2:
HeLa
Positive Control 3:
HT-1080
Positive Control 4:
Mouse kidney
Positive Control 5:
Mouse spinal cord
Positive Control 6:
Mouse testis
Predicted Molecular Weight:
Observed: 113kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
ANKRD28
Additional Names:
PITK, PPP1R65, serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit A, PP6-ARS-A, ankyrin repeat domain-containing protein 28, phosphatase interactor targeting K protein, phosphatase interactor targeting protein hnRNP K, protein phosphatase 1, regulatory subunit 65, protein phosphatase 6 ankyrin repeat subunit A, serine/threonine-protein phosphatase 6 regulatory subunit ARS-A
Ncbi Gene Id Number:
23243
User Note:
Optimal dilutions for each application to be determined by the researcher.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-537

List Size:
100 uL

List Price:

$436.00

Datasheet
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