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Western blot analysis of extracts of various cell lines, using ADCY1 antibody (23-902) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Western blot analysis of extracts of various cell lines, using ADCY1 antibody (23-902) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.

ADCY1 Antibody

CATALOG NUMBER: 23-902

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 470-610 of human ADCY1 (NP_066939.1).
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
WB: 1:200 - 1:1000
Positive Control 1:
mouse brain
Positive Control 2:
mouse kidney
Predicted Molecular Weight:
Observed: 123kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
ADCY1
Additional Names:
AC1, DFNB44, adenylate cyclase type 1, 3',5'-cyclic AMP synthetase, ATP pyrophosphate-lyase 1, Ca(2+)/calmodulin-activated adenylyl cyclase, adenyl cyclase, adenylate cyclase 1 (brain), adenylate cyclase type I, adenylyl cyclase 1
Ncbi Gene Id Number:
107
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the of adenylate cyclase gene family that is primarily expressed in the brain. This protein is regulated by calcium/calmodulin concentration and may be involved in brain development. Alternate splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-902

List Size:
100 uL

List Price:

$436.00

Out of stock

Datasheet
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