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Western blot analysis of extracts of various cell lines, using WDR77 antibody (23-974) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Western blot analysis of extracts of various cell lines, using WDR77 antibody (23-974) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

WDR77 Antibody [8B2F7]

CATALOG NUMBER: 23-974

Clonality:
Monoclonal
Tested Applications:
ICC, WB
Host Species:
Mouse
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein of human WDR77
Conjugate:
Unconjugated
Tested Applications:
ICC, WB
Application Note:
WB: 1:500 - 1:2000
ICC: 1:50 - 1:200
Positive Control 1:
K-562
Positive Control 2:
HeLa
Positive Control 3:
293T
Positive Control 4:
Jurkat
Positive Control 5:
HepG2
Positive Control 6:
MCF7
Predicted Molecular Weight:
Observed: 39kDa
Purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
WDR77
Additional Names:
WDR77, Androgen receptor cofactor p44, HKMT1069, MEP-50, Methylosome protein 50, p44, MEP50, Nbla10071, RP11-552M11.3, WD repeat domain 77
Ncbi Gene Id Number:
79084
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is an androgen receptor coactivator that forms a complex with protein arginine methyltransferase 5, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins. The encoded protein may be involved in the early stages of prostate cancer, with most of the protein being nuclear-localized in benign cells but cytoplasmic in cancer cells. Several transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-974

List Size:
100 uL

List Price:

$540.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

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