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Western blot analysis of extracts of various cell lines, using ADAM10 antibody (24-024) at 1:1000 dilution.<br />Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.<br />Lysates/proteins: 25ug per lane.<br />Blocking buffer: 3% nonfat dry milk in TBST.<br />Detection: ECL Basic Kit<br />Exposure time: 30s.
Immunofluorescence analysis of HeLa cells using ADAM10 antibody (24-024) at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using ADAM10 antibody (24-024) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit
Exposure time: 30s.

ADAM10 Antibody

CATALOG NUMBER: 24-024

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 214-500 of human ADAM10 (NP_001101.1).
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
WB 1:500 - 1:2000
IF 1:50 - 1:200
Positive Control 1:
HT-1080
Positive Control 2:
Raji
Positive Control 3:
Jurkat
Positive Control 4:
Mouse Kidney
Positive Control 5:
Mouse brain
Predicted Molecular Weight:
Observed: 70kDa, 105 kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
ADAM10
Additional Names:
ADAM10 Antibody: RAK, kuz, AD10, AD18, MADM, CD156c, HsT18717, KUZ, Disintegrin and metalloproteinase domain-containing protein 10, CDw156, ADAM 10
Ncbi Gene Id Number:
102
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. Alternate splicing results in multiple transcript variants encoding different proteins that may undergo similar processing.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

24-024

List Size:
100 uL

List Price:

$436.00

Datasheet
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