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<strong>Figure 1 Western Blot Validation in Human Cell Lines and Mouse and Rat Tissues</strong><br>Loading: 25 µg of lysates per lane. Antibodies: TMPRSS2 antibody, 24-034, at 1:1000 dilution. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Blocking buffer: 3% nonfat dry milk in TBST.
<strong>Figure 2 Immunohistochemistry Validation of TMPRSS2 in Human Esophageal Tissue</strong><br> Immunohistochemistry of paraffin-embedded human esophageal tissue using TMPRSS2 antibody, 24-034, at 1:100 dilution (40x lens).
Figure 1 Western Blot Validation in Human Cell Lines and Mouse and Rat Tissues
Loading: 25 µg of lysates per lane. Antibodies: TMPRSS2 antibody, 24-034, at 1:1000 dilution. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Blocking buffer: 3% nonfat dry milk in TBST.

TMPRSS2 Antibody [ARC2373]

CATALOG NUMBER: 24-034

Clonality:
Monoclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Virus
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Virus
Immunogen:
Synthetic Peptide
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000, IHC: 1:50 - 1:200
Purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH 7.3
Concentration:
batch dependent
Storage Conditions:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Ncbi Official Symbol:
TMPRSS2
Additional Names:
PP9284, PRSS10, transmembrane serine protease 2
Protein Accession Number:
O15393
Ncbi Gene Id Number:
7113
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a protein that belongs to the serine protease family. The encoded protein contains a type II transmembrane domain, a receptor class A domain, a scavenger receptor cysteine-rich domain and a protease domain. Serine proteases are known to be involved in many physiological and pathological processes. This gene was demonstrated to be up-regulated by androgenic hormones in prostate cancer cells and down-regulated in androgen-independent prostate cancer tissue. The protease domain of this protein is thought to be cleaved and secreted into cell media after autocleavage. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

24-034

List Size:
100 ul

List Price:

$540.00

Datasheet
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