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Western Blot at 1:500 dilution Lane 1: Hela whole cell lysate Lane 2: mouse brain lysate Lane 3: mouse heart lysate Lane 4: PANC-1 whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: human heart lysate Lane 2: PANC-1 whole cell lysate Lane 3: mouse heart lysate Lane 4: rat heart lysate Lysates/proteins at 20 ug per lane.
WNT5A Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western Blot at 1:500 dilution Lane 1: Hela whole cell lysate Lane 2: mouse brain lysate Lane 3: mouse heart lysate Lane 4: PANC-1 whole cell lysate Lysates/proteins at 20 ug per lane.

WNT5A Antibody

CATALOG NUMBER: 62-006

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Rabbit
Immunogen:
This WNT5A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 185-213 amino acids from the Central region of human WNT5A.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:500
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
42 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
WNT5A
Additional Names:
Protein Wnt-5a, WNT5A
Protein Accession Number:
P41221
Protein Gi Number:
212276478
Ncbi Gene Id Number:
7474
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The WNT gene family consists of structurally related geneswhich encode secreted signaling proteins. These proteins have beenimplicated in oncogenesis and in several developmental processes,including regulation of cell fate and patterning duringembryogenesis. This gene is a member of the WNT gene family. Itencodes a protein which shows 98%, 98% and 87% amino acid identityto the mouse, rat and the xenopus Wnt5A protein, respectively. Theexperiments performed in Xenopus laevis embryos identified thathuman frizzled-5 (hFz5) is the receptor for the Wnt5A ligand andthe Wnt5A/hFz5 signaling mediates axis induction. [provided byRefSeq].
Background Reference 1:
He, X., et al. Science 275(5306):1652-1654(1997)
Background Reference 2:
Danielson, K.G., et al. J. Biol. Chem. 270(52):31225-31234(1995)
Background Reference 3:
Smolich, B.D., et al. Mol. Biol. Cell 4(12):1267-1275(1993)
Background Reference 4:
Clark, C.C., et al. Genomics 18(2):249-260(1993)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-006

List Size:
200 ul

List Price:

$495.00

Datasheet
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