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Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in mouse brain tissue and HepG2,293 cell line lysates (35ug/lane).
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

FBXO2 Antibody

CATALOG NUMBER: 55-088

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This FBXO2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 244-271 amino acids from the C-terminal region of human FBXO2.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For FACS starting dilution is: 1:10~50
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
33 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
FBXO2
Additional Names:
F-box only protein 2, FBXO2, FBX2
Protein Accession Number:
Q9UK22
Protein Gi Number:
51338836
Ncbi Gene Id Number:
26232
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. This protein is highly similar to the rat NFB42 (neural F Box 42 kDa) protein which is enriched in the nervous system and may play a role in maintaining neurons in a postmitotic state.
Background Reference 1:
Eom, C.Y., et al. Proc. Natl. Acad. Sci. U.S.A. 100(17):9803-9807(2003) Ilyin, G.P., et al. Gene 296 (1-2), 11-20 (2002)Yoshida, Y., et al. Nature 418(6896):438-442(2002) Winston, J.T., et al. Curr. Biol. 9(20):1180-1182(1999) Cenciarelli, C., et al. Curr. Biol. 9(20):1177-1179(1999)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-088

List Size:
400 ul

List Price:

$495.00

Datasheet
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