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Western blot analysis in WiDr cell line lysates (35ug/lane).
Western blot analysis in WiDr cell line lysates (35ug/lane).

BARD1 Antibody

CATALOG NUMBER: 55-322

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This BARD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 101-131 amino acids from the N-terminal region of human BARD1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
87 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
BARD1
Additional Names:
BRCA1-associated RING domain protein 1, BARD-1, 632-, BARD1
Protein Accession Number:
Q99728
Protein Gi Number:
116241265
Ncbi Gene Id Number:
580
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
BARD1 is a protein which interacts with theN-terminal region of BRCA1. In addition to its ability to bindBRCA1 in vivo and in vitro, it shares homology with the 2 mostconserved regions of BRCA1: the N-terminal RING motif and theC-terminal BRCT domain. The RING motif is a cysteine-rich sequencefound in a variety of proteins that regulate cell growth, includingthe products of tumor suppressor genes and dominant protooncogenes.This protein also contains 3 tandem ankyrin repeats. TheBARD1/BRCA1 interaction is disrupted by tumorigenic amino acidsubstitutions in BRCA1, implying that the formation of a stablecomplex between these proteins may be an essential aspect of BRCA1tumor suppression. This protein may be the target of oncogenicmutations in breast or ovarian cancer.
Background Reference 1:
Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263(2010)
Background Reference 2:
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Background Reference 3:
Dizin, E., et al. Int. J. Biochem. Cell Biol. 42(5):693-700(2010)
Background Reference 4:
Irminger-Finger, I. Gynecol. Oncol. 117(2):211-215(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-322

List Size:
400 ul

List Price:

$495.00

Datasheet
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