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Western blot analysis in CEM cell line lysates (35ug/lane).
Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in CEM cell line lysates (35ug/lane).

AT8B2 Antibody

CATALOG NUMBER: 55-414

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This AT8B2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 18-47 amino acids from the N-terminal region of human AT8B2.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
137 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ATP8B2
Additional Names:
Phospholipid-transporting ATPase ID, ATPase class I type 8B member 2, P4-ATPase flippase complex alpha subunit ATP8B2, ATP8B2, ATPID, KIAA1137
Protein Accession Number:
P98198
Protein Gi Number:
30316371
Ncbi Gene Id Number:
57198
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene belongs to the family ofP-type cation transport ATPases, and to the subfamily ofaminophospholipid-transporting ATPases. The aminophospholipidtranslocases transport phosphatidylserine andphosphatidylethanolamine from one side of a bilayer to another.Alternatively spliced transcript variants encoding differentisoforms have been identified.
Background Reference 1:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 2:
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Background Reference 3:
Harris, M.J., et al. Biochim. Biophys. Acta 1633(2):127-131(2003)
Background Reference 4:
Halleck, M.S., et al. Physiol. Genomics 1(3):139-150(1999)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-414

List Size:
400 ul

List Price:

$495.00

Datasheet
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