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Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
ACD10 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).

ACD10 Antibody

CATALOG NUMBER: 55-435

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This ACD10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 393-421 amino acids from the Central region of human ACD10.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
119 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ACAD10
Additional Names:
Acyl-CoA dehydrogenase family member 10, ACAD-10, 1399-, ACAD10
Protein Accession Number:
Q6JQN1
Protein Gi Number:
74748862
Ncbi Gene Id Number:
80724
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the acyl-CoA dehydrogenasefamily of enzymes (ACADs), which participate in the beta-oxidationof fatty acids in mitochondria. The encoded enzyme contains ahydrolase domain at the N-terminal portion, a serine/threonineprotein kinase catlytic domain in the central region, and aconserved ACAD domain at the C-terminus. Several alternativelyspliced transcript variants of this gene have been described, butthe full-length nature of some of these variants has not beendetermined.
Background Reference 1:
Bian, L., et al. Diabetologia 53(7):1349-1353(2010)
Background Reference 2:
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Background Reference 3:
Vega, A., et al. Gynecol. Oncol. 112(1):210-214(2009)
Background Reference 4:
Ye, X., et al. Mol. Biol. Rep. 31(3):191-195(2004)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-435

List Size:
400 ul

List Price:

$495.00

Datasheet
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