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Western blot analysis in HL-60 cell line lysates (35ug/lane).
BCORL1 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in HL-60 cell line lysates (35ug/lane).

BCORL1 Antibody

CATALOG NUMBER: 55-463

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This BCORL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 69-98 amino acids from the N-terminal region of human BCORL1.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
183 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
BCORL1
Additional Names:
BCL-6 corepressor-like protein 1, BCoR-L1, BCoR-like protein 1, BCORL1
Protein Accession Number:
Q5H9F3
Protein Gi Number:
74762178
Ncbi Gene Id Number:
63035
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a transcriptionalcorepressor that is found tethered to promoter regions byDNA-binding proteins. The encoded protein can interact with severaldifferent class II histone deacetylases to repress transcription.Two transcript variants encoding different isoforms have been foundfor this gene.
Background Reference 1:
Pagan, J.K., et al. J. Biol. Chem. 282(20):15248-15257(2007)
Background Reference 2:
Lose, F., et al. Breast Cancer Res.(4), R54 (2007) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-463

List Size:
400 ul

List Price:

$495.00

Datasheet
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