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Western blot analysis in ZR-75-1 cell line lysates (35ug/lane).
Western blot analysis in CHO cell line lysates (35ug/lane).
ASAP3 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in ZR-75-1 cell line lysates (35ug/lane).

ASAP3 Antibody

CATALOG NUMBER: 55-552

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Hamster, Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Hamster, Human
Immunogen:
This ASAP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 26-54 amino acids from the N-terminal region of human ASAP3.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
99 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ASAP3
Additional Names:
Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 3, Development and differentiation-enhancing factor-like 1, Protein up-regulated in liver cancer 1, ASAP3, DDEFL1, UPLC1
Protein Accession Number:
Q8TDY4
Protein Gi Number:
74751433
Ncbi Gene Id Number:
55616
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of a subfamily ofADP-ribosylation factor(Arf) GTPase-activating proteins thatcontain additional ankyrin repeat and pleckstrin homology domains.The Arf GAP domain of this protein catalyzes the hydrolysis of GTPbound to Arf proteins. The encoded protein promotes celldifferentiation and migration and has been implicated in cancercell invasion. Alternative splicing results in multiple transcriptvariants.
Background Reference 1:
Ismail, S.A., et al. Cell 141(5):812-821(2010)
Background Reference 2:
Ha, V.L., et al. J. Biol. Chem. 283(22):14915-14926(2008)
Background Reference 3:
Fang, Z., et al. Mol. Cell Proteomics 5(8):1437-1449(2006)
Background Reference 4:
Okabe, H., et al. Int. J. Oncol. 24(1):43-48(2004)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-552

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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