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Western Blot at 1:1000 dilution + HUVEC whole cell lysate Lysates/proteins at 20 ug per lane.
CAF-1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Fluorescent confocal image of Hela cell stained with CAF-1 Antibody (N-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CAF-1 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). CAF-1 immunoreactivity is localized to Cytoplasm and Nucleus significantly.
Western Blot at 1:1000 dilution + HUVEC whole cell lysate Lysates/proteins at 20 ug per lane.

CAF-1 Antibody

CATALOG NUMBER: 55-638

Clonality:
Polyclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Chicken, Zebrafish, Mouse, Xenopus
Immunogen:
This CAF-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 34-61 amino acids from the N-terminal region of human CAF-1.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
33 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CNOT7
Additional Names:
CCR4-NOT transcription complex subunit 7, BTG1-binding factor 1, CCR4-associated factor 1, CAF-1, Caf1a, CNOT7, CAF1
Protein Accession Number:
Q9UIV1
Protein Gi Number:
41713629
Ncbi Gene Id Number:
29883
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene binds to ananti-proliferative protein, B-cell translocation protein 1, whichnegatively regulates cell proliferation. Binding of the twoproteins, which is driven by phosphorylation of theanti-proliferative protein, causes signaling events in celldivision that lead to changes in cell proliferation associated withcell-cell contact. The protein has both mouse and yeast orthologs.Alternate splicing of this gene results in two transcript variantsencoding different isoforms.
Background Reference 1:
Lau, N.C., et al. Biochem. J. 422(3):443-453(2009)
Background Reference 2:
Aslam, A., et al. Mol. Biol. Cell 20(17):3840-3850(2009)
Background Reference 3:
Miyasaka, T., et al. Cancer Sci. 99(4):755-761(2008)
Background Reference 4:
Nishida, K., et al. Acta Crystallogr. Sect.Struct. Biol. Cryst. Commun. 63 (PT 12), 1061-1063 (2007) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-638

List Size:
400 ul

List Price:

$495.00

Datasheet
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