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Western blot analysis in ZR-75-1 cell line lysates (35ug/lane).
Western blot analysis in mouse spleen tissue lysates (35ug/lane).
ACAP1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in ZR-75-1 cell line lysates (35ug/lane).

ACAP1 Antibody

CATALOG NUMBER: 55-658

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This ACAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 96-125 amino acids from the N-terminal region of human ACAP1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
82 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ACAP1
Additional Names:
Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1, Centaurin-beta-1, Cnt-b1, ACAP1, CENTB1, KIAA0050
Protein Accession Number:
Q15027
Protein Gi Number:
3183210
Ncbi Gene Id Number:
9744
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Catalyzes the transfer of Gal to GlcNAc-based acceptors with a preference for the core3 O-linked glycan GlcNAc(beta1,3)GalNAc structure. Can use glycolipid LC3Cer as an efficient acceptor.
Background Reference 1:
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Background Reference 2:
Cuthbert, E.J., et al. Am. J. Physiol., Cell Physiol. 294 (1), C263-C270 (2008) :
Background Reference 3:
Li, J., et al. J. Cell Biol. 178(3):453-464(2007)
Background Reference 4:
Wu, C., et al. Proteomics 7(11):1775-1785(2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-658

List Size:
400 ul

List Price:

$495.00

Datasheet
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