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Western blot analysis in mouse liver tissue lysates (35ug/lane).
AHCY Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in mouse liver tissue lysates (35ug/lane).

AHCY Antibody

CATALOG NUMBER: 55-701

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Monkey, Pig
Immunogen:
This AHCY antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 79-110 amino acids from the N-terminal region of human AHCY.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
48 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
AHCY
Additional Names:
Adenosylhomocysteinase, AdoHcyase, S-adenosyl-L-homocysteine hydrolase, AHCY, SAHH
Protein Accession Number:
P23526
Protein Gi Number:
20141702
Ncbi Gene Id Number:
191
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
S-adenosylhomocysteine hydrolase belongs to theadenosylhomocysteinase family. It catalyzes the reversiblehydrolysis of S-adenosylhomocysteine (AdoHcy) to adenosine (Ado)and L-homocysteine (Hcy). Thus, it regulates the intracellularS-adenosylhomocysteine (SAH) concentration thought to be importantfor transmethylation reactions. Deficiency in this protein is oneof the different causes of hypermethioninemia. Alternativelyspliced transcript variants encoding different isoforms have beenfound for this gene.
Background Reference 1:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 2:
Gass, N., et al.Affect Disord 126 (1-2), 134-139 (2010) :
Background Reference 3:
Giusti, B., et al. Thromb. Haemost. 104(2):231-242(2010)
Background Reference 4:
Levine, A.J., et al. Cancer Epidemiol. Biomarkers Prev. 19(7):1812-1821(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-701

List Size:
400 ul

List Price:

$495.00

Datasheet
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