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Western blot analysis in CEM cell line lysates (35ug/lane).
Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in CEM cell line lysates (35ug/lane).

DUOX2 Antibody

CATALOG NUMBER: 55-718

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This DUOX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 513-542 amino acids from the Central region of human DUOX2.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
175 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DUOX2
Additional Names:
Dual oxidase 2, 1111-, Large NOX 2, Long NOX 2, NADH/NADPH thyroid oxidase p138-tox, NADPH oxidase/peroxidase DUOX2, NADPH thyroid oxidase 2, Thyroid oxidase 2, p138 thyroid oxidase, DUOX2, LNOX2, THOX2
Protein Accession Number:
Q9NRD8
Protein Gi Number:
296434485
Ncbi Gene Id Number:
50506
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a glycoprotein and amember of the NADPH oxidase family. The synthesis of thyroidhormone is catalyzed by a protein complex located at the apicalmembrane of thyroid follicular cells. This complex contains aniodide transporter, thyroperoxidase, and a peroxide generatingsystem that includes this encoded protein and DUOX1. This proteinis known as dual oxidase because it has both a peroxidase homologydomain and a gp91phox domain.
Background Reference 1:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 2:
Salmeen, A., et al. Oncogene 29(31):4473-4484(2010)
Background Reference 3:
Linderholm, A.L., et al. Am. J. Physiol. Lung Cell Mol. Physiol. 299 (2), L215-L221 (2010) :
Background Reference 4:
Roberts, K.E., et al. Gastroenterology 139(1):130-139(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-718

List Size:
400 ul

List Price:

$495.00

Datasheet
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